摘要
目的观察生理盐水、亚胺培南、氟康唑支气管肺泡灌洗后对兔肺泡内炎症因子的影响。方法新西兰白兔34只气管插管后分为四组:A组(3只)作为空白对照;B组(11只)用生理盐水支气管肺泡灌洗;C组(8只)用亚胺培南灌洗;D组(12只)用氟康唑灌洗。2h后处死动物,ELISA法比较肺泡灌洗液及肺组织匀浆中肿瘤坏死因子α(TNF-α)含量。结果四组肺泡灌洗液的TNF-α水平各组间差异无统计学意义(P>0.05)。C组肺组织匀浆TNF-α水平低于A、B、D组(P<0.05)。结论生理盐水、亚胺培南、氟康唑支气管肺泡灌洗并未增加局部TNF-α的释放。
Objective To investigate the effect of bronchoalveolar lavage（BAL） on the expression of tumor necrosis factor-α（TNF-α） in rabbit alveolar.Methods After tracheal intubation,34 New Zealand rabbits were randomly divided into 4 groups of A（n=3,as the blank control）,B（n=11,BAL with normal saline）,C（n=8,BAL with imipenem）,and D（n=12,BAL with fluconazol）.The rabbits were sacrificed at 2 hours after BAL.The concentrations of TNF-α in bronchoalveolar lavage fluids（BALF） and lung tissue homogenate were determined by ELISA.Results The concentrations of TNF-α in BALF showed no significant difference among four groups（P0.05）.But the concentration of TNF-α in lung tissue homogenate was lower in group C than that in the other three groups（P0.05）.Conclusion BAL with normal saline,imipenem or fluconazol does not increase the expression of TNF-α in BALF of rabbits.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第16期1928-1929,共2页
Jiangsu Medical Journal
