金银花脂溶性成分的气相色谱-质谱分析

GC-MS Analysis of Liposoluble Contents from Flos Lonicerae Japonicae

目的分析金银花脂溶性化学成分,为其质量评价提供科学依据。方法采用索氏提取法提取脂溶性成分,用气相毛细管柱进行分析,归一化法测定其相对含量,并用气象色谱-质谱(GC-MS)法鉴定其化学成分。结果检出39个色谱峰,鉴定出29个化合物,占总组分的94.03%。结论金银花脂溶性成分主要为烃类和脂肪酸,其中13种烃类化合物占总脂溶性成分的45.10%,11种脂肪酸占总脂溶性成分的43.18%;植物甾醇为次要成分,占总脂溶性成分的8.26%。

Objective To analyze the chemical constituents of the liposoluble contents from Flos Lonicerae japonicae and to provide scientific foundations on quality evaluation. Methods The liposoluble contents of Flos Lonicerae japonicae were extracted by Soxet extraction and separated with GC capillary column. The relative content of every compound was determined by normalization method and the chemical compositions were evaluated by GC-MS. Results Thirty-nine chromatographic peaks were isolated and twenty-nine compounds were identified, accounting for 94.03% of the total liposoluble contents in Flos Lonicerae japonicae. Conclusion The essential components of Flos Lonicerae japonicae are hydrocarbon compounds and fatty acid, 45.10% of the total liposoluble contents are 13 kinds of hydrocarbon compounds, 43.18% of the total liposoluble contents are 11 kinds of fatty acid. Phytosterol was the secondary composition, accounting for 8.26%.