草鱼血清凝集素的研究

Study on an Agglutinin Isolated from Serum of Grass Carp (Ctenopharyngodon idellus)

从草鱼血清分离到一种天然的凝集素分子，能够凝集多种不同的抗原物质，其组成成分为糖蛋白，具有耐热和耐酸碱性，对β－巯基乙醇敏感．凝集反应受温度、反应介质的ｐＨ、离子强度以及时间等因素的影响．经糖的专一性抑制实验显示，半乳糖和乳糖对凝集反应的抑制作用最强，甘露糖和Ｎ－乙酰葡萄糖胺也有一定的抑制作用。

A natural occuring agglutinin, which was detected from serum of grass carp(Ctenopharyngodon idellus), was found to have the ability of agglutinating different kinds of antigen. It has the characteristic of glycoprotein.The agglutinin was stable when exposure to the heat (60℃) for 1h and acid(pH=2) or alkaline(pH=10) for 24h respectively, but was sensitive to the treatment of 1% β-Mercapto ethanol, its agglutinating activity was influenced by the temperature , concentration of ion , reaction time and pH value of the buffer.The agglutinating activity could be inhibited by the splitting of sugar and lactose, galoctose acetylglucosamine and mannose respectively, the results demonstrated the sugar refered above played an important role in agglutinating reaction.