摘要
从生物硝化池污水水样中分离了硝化细菌ＤＮＡ，以其为模板，针对其１６ＳｒＲＮＡ基因及２３ＳｒＲＮＡ基因而设计合成了两对引物并分别进行了ＰＣＲ扩增，扩增产物的２％琼脂糖凝胶电泳结果显示，硝化细菌ＤＮＡ模板得到了特异性扩增．参照分子量标准（Ｍａｒｋｅｒ）的电泳结果，被扩增的ＤＮＡ条带大小分别约为４００ｂｐ和７３０ｂｐ．
Nitrobacteria DNA was extracted from wastewater of biological nitrification stage and two PCR primers based on the DNA sequences of 16S rDNA and 23S rDNA were synthesized and used for PCR amplification. The results showed that Nitrobacteria DNA was amplified specifically and the size of amplified DNA fragments were about 400 bp and 730 bp respectively. It is suggested that PCR analysis can be used for the rapid detection of Nitrobacteria in wastewater.
出处
《浙江大学学报（理学版）》
CAS
CSCD
1999年第2期83-86,共4页
Journal of Zhejiang University（Science Edition）
基金
浙江省教委和浙江省环保局资助项目
