非小细胞肺癌组织Survivin和K-ras基因蛋白表达及其与放疗敏感相关性的探讨

Association of Survivin and K-ras protein with radiosensitivity in non-small cell lung cancer tissues

目的:研究生存素(Survivin)、K-ras蛋白表达与非小细胞肺癌(NSCLC)放疗敏感性的关系,为NSCLC放疗敏感性的预测提供依据。方法:采用免疫组化染色法检测62例晚期NSCLC的Sur-vivin、K-ras蛋白的表达,所有病例均给予总量为60～70Gy的三维适形放疗,按照实体瘤近期疗效标准(RECIST标准)进行疗效评价,分析Survivin、K-ras蛋白的表达与放疗敏感性的关系。结果:Sur-vivin蛋白(-)、(+)、(++)和(+++)患者经过放疗后,获得近期治疗有效率分别为94.1%、92.9%、80.0%和68.8%,组间比较差异有统计学意义,χ2=4.578,P=0.032;K-ras蛋白(-)、(+)、(++)和(+++)患者经过放疗后,获得近期治疗有效率分别为93.8%、89.5%、81.3%和63.6%,组间比较差异有统计学意义,χ2=4.439,P=0.035。结论:Survivin、K-ras蛋白表达与NSCLC患者的近期放疗疗效呈明显的负相关,检测这两种蛋白的表达有望成为预测NSCLC放疗敏感性的指标。

OBJECTIVE：To investigate the expression of Survivin,K-ras protein in non-small cell lung cancer （NSCLC） and its relationship to the effect of radiotherapy,and analyze the possibility of Survivin and K-ras gene as molecular markers to predict the radiosensitivity of NSCLC.METHODS：The expression of Survivin and K-ras protein was detected by immunohistochemistry in 62 specimens of advanced NSCLC.All patients received total 60-70 Gy of 3D-radiotherapy.The association of Survivin and K-ras protein expression with the effect of radiotherapy was analyzed.RESULTS：The short-time effect rates of Survivin（-）,（＋）,（＋＋） and （＋＋＋） were 94.1%,92.9%,80.0% and 68.8%（χ^2=4.578,P=0.032）,while those of K-ras were 93.8%,89.5%,81.3% and 63.6%（χ^2=4.439,P=0.035）.CONCLUSIONS：The expression of Survivin and K-ras protein is related to radiosensitivity in NSCLC.The expression of Survivin and K-ras may be useful markers to predict the radiosensitivity of NSCLC.