肝癌细胞系中DNA甲基转移酶和HLAⅠ类分子的表达及意义

Expressions of three DNA methyltransferases and classⅠHLA in hepatocellular carcinoma cells

目的:探讨甲基转移酶(DNMT)3a及HLAⅠ类分子在肝癌细胞系中表达及意义。方法:提取HepG2、MHCC97L、MHCC97M3、SMMC7421和BEL7402的mRNA,应用RT-PCR及实时定量PCR检测DNMT及HLA的表达情况。结果:RT-PCR显示,在5个肝癌细胞系中DN-MT1和DNMT3b均高表达。而DNMT3a在高转移的MHCC97M3中的表达低于其他细胞。另外,HLA-A、HLA-B和HLA-C则普遍低表达。定量PCR示,DNMT3a在MHCC97L中的相对表达量为1,在MHCC97M3中为0.33。同时,前者的HLA-A、HLA-B和HLA-C的相对表达量分别为4、4.199和3.9,而在后者中分别是2.129、2.621和2.55。结论:在相同的遗传背景下,DNMT3a和HLAⅠ类分子的高表达与肝癌细胞系的低转移能力相关。

OBJECTIVE：To analyse the correlation betweeb the metastasis and expressions of three DNA methyltransferases （DNMT） and classⅠHuman Leukocyte Antigens（HLA） mRNA in hepatocellular carcinoma（HCC） cell lines.METHODS：mRNAs of HepG2,MHCC97L,MHCC97M3,SMMC7421,and BEL7402 cells were isolated.RT-PCR and Real-Time Quantitative PCR were used to detect the expressions of DNMTs and HLAs molecules at the mRNA level.RESULTS：RT-PCR showed,overexpressions of DNMT1 and DNMT3b were simultaneously presented in all the five HCC cells.But expression of DNMT3a was lower in MHCC97M3 that was the higher potential of lung-metastasis,than that in other cells.HLA-A,HLA-B and HLA-C expressions were low expressed in these cells.In Real-Time quantitative PCR,the realtive expression of DNMT3a was 1 in MHCC97L cells,and 0.33 in MHCC97M3 cells.Meanwhile,the relative expressions of HLA,HLA-B and HLA-C were 4,4.199 and 3.9 respectively in the former,and 2.129,2.621 and 2.55 in the latter.CONCLUSION：DNMT3a and HLAⅠmolecules are correlated with the lower metastasis potential of HCC cell lines with the same genetic condition.