摘要
目的建立大鼠吸入全氟异丁烯（perfluoroisobutylene，PFIB）染毒致急性肺损伤实验模型，并进行血液和组织中细胞因子和组织形态学改变观察。方法制作大鼠吸入PFIB诱发急性肺损伤动物模型，测定动物染毒后肺灌洗液（BALF）中总蛋白含量和肺含水量变化，血清和肺组织中白介素-1（IL-1）、白介素-8（IL-8）、白介素-10（IL-10）、白介素-18（IL-18）和肿瘤坏死因子-α（TNF-α）含量改变，以及肺组织病理学观察。结果（1）模型大鼠肺含水量和BALF蛋白含量较空白对照组明显增加；（2）PFIB染毒诱发急性肺损伤动物血清和肺组织中IL-1，8、10、18和TNF—α细胞因子可见特征性改变；（3）形态学观察可见肺组织水肿和明显的炎症反应。结论研究和探讨PFIB吸入诱发急性肺损伤的变化规律和特点可提供更多的急性肺损伤预防和治疗信息。
Objective To establish the model of acute lung injury（ALI） of perfluoroisobutylene （PFIB） inhalation and to study the change of the cell factor in blood and lungs tissue, pathological changes of the lung tissue. Methods The model of acute lung injury was induced by （PFIB） inhalation. The protein concentration in bronchoalveolar lavage fluid （BALF）, the liquid volume of lung, and the cell factor of interleukin-1. 8, 10, 18 and TNF-α were assessed. The pathology of lung injury were examined. Rusult （1）In the model rats, the liquid volume of lung, and BALF protein concentration are higher than those of negative group. （2） In the acute lung injury rats of PFIB, the characteristic changes of the interleukin -1. 8. 10, 18 and TNF-α in blood and lungs tissue were deteted; （3） The pathology shows that the changes of pulmonary edema and the inflammayory reaction of ALI. Conclusion The research and exploration on the changes and regularity of ALI induced by PFIB inhalation can provide more information about ALI protection and treatment.
出处
《实验动物与比较医学》
CAS
2010年第4期257-260,共4页
Laboratory Animal and Comparative Medicine
基金
军队医药卫生科研科学基金一面上项目（基金编号06MB076）
