蛋白酶激活受体-2在原发性肝癌组织中的表达

Expression of proteinase-activated receptor-2 in primary hepatic carcinoma

目的:研究蛋白酶激活受体-2(PAR-2)在原发性肝癌(PHC)中的蛋白表达水平,并探讨其临床价值.方法:采用免疫组织化学和免疫荧光技术检测41例PHC患者癌组织和癌旁正常组织中PAR-2的蛋白表达水平.结果:PAR-2在PHC组织及癌旁正常组织中均有不同程度的表达,癌旁组织主要表现为核周胞质染色,而肝癌组织呈弥漫性胞质染色.PHC与癌旁组织的表达差异具有显著性意义(92.67±8.53vs57.52±7.31,P<0.05);不同组织学类型的肝癌表达不均衡,肝细胞癌高于胆管细胞癌,但差异无统计学意义(141.05±18.36vs112.10±10.05,P>0.05);肿瘤转移者高于无转移者,差异有统计学意义(167.83±8.91vs73.25±4.05,P<0.05).结论:PAR-2在PHC中高表达;PAR-2的表达与PHC的发生和发展密切相关.

AIM：To investigate the expression of proteinaseactivated receptor-2（PAR-2） protein in primary hepatic carcinoma（PHC） and to evaluate its clinical value. METHODS：Immunohistochemistry and immunofluorescence were used to examine the expression of PAR-2 protein in cancer tissue and cancer-adjacent normal tissue from 41 patients with PHC. RESULTS：The expression pattern of PAR-2 protein was different between cancer tissue and cancer-adjacent normal tissue.Perinuclear cytoplasmic staining was noted in normal tissue,while cancer tissue showed diffuse cytoplasmic staining.PAR-2 labeling index was signif icantly higher in cancer tissue than in normal tissue（92.67 ± 8.53 vs 57.52 ± 7.31,P〈 0.05）.Although PAR-2 labeling index was higher in hepatocarcinoma than in cholangiocarcinoma,no statistical signif icance was observed in PAR-2 labeling index between them（141.05 ± 18.36 vs 112.10 ± 10.05,P〉 0.05）.PAR-2 labeling index was significantly higher in patients with cancer metastasis than in those without cancer metastasis（167.83 ± 8.91 vs 73.25 ± 4.05,P〈 0.05）. CONCLUSION：PAR-2 is over-expressed in PHC.PAR-2 expression is closely related with the development and progression of PHC.