摘要
目的:研究口服避孕药达英-35在雌性大鼠中减轻环磷酰胺(CTX)诱导的性腺毒性的效果。方法:随机将75只雌性Wistar大鼠分为5组:空白对照组、CTX组、达英-35组、联合治疗Ⅰ组和联合治疗Ⅱ组。用放射免疫法测定各组雌二醇(E2)和卵泡刺激素(FSH)水平,并于停药当天、第15天、30天,处死各组1/3的大鼠,比较卵巢重量、卵泡数量变化。结果:停药第30天,CTX组大鼠E2为(21.88±2.10)pg/ml,明显低于两个联合治疗组,FSH为(7.05±0.18)mIU/ml,明显高于两个联合治疗组,差异均有统计学意义(P<0.05);CTX组卵巢重量和卵泡总数分别为(32.40±5.55)mg和308±32个,明显低于两个联合治疗组,差异有统计学意义(P<0.05);联合治疗Ⅰ、Ⅱ组的E2、FSH、卵巢重量、卵泡数量分别比较,差异无统计学意义(P>0.05)。结论:达英-35降低了CTX对大鼠卵巢功能的损伤,对卵巢功能的保护和恢复起到了一定的作用。
Objective：To study the effect of oral contraceptives（OC） Diane-35 against cyclophosphamide（CTX）induced gonadotoxicity in the female rats.Methods：75 female Wistar rats were divided into five groups：blank control group,CTX group,Diane-35 group,combination groupⅠ,combination groupⅡ.E2,FSH were measured by RIA,and one-third of rats in each group were killed respectively on the stop day,15 days,30 days to compare the weight of ovary and the number of follicles.Results：On stop 30 days,E2 of CTX group was （21.88±2.10）pg/ml,lower than that of combination groups（P0.05）,FSH was（7.05±0.18）mIU/ml,higher than that of combination groups（P0.05）,ovary weight and total follicle number of CTX group were （32.40±5.55）mg and 308±32,obviously lower than those of combination groups（P0.05）.No significant differences were found between the two combination groups in E2,FSH,ovary weight and follicle number（P0.05）.Conclusion：Diane-35 may decrease CTX-induced ovarian damage in rat and plays a potential role in protecting and restoring ovarian function.
出处
《现代妇产科进展》
CSCD
北大核心
2010年第7期498-501,共4页
Progress in Obstetrics and Gynecology
