细菌外毒素序列中特有模体的识别及其基因本体注释分析

Identification of exotoxin-specific motifs/domains in bacterial exotoxin sequences and corresponding Gene Ontology analysis

【目的】识别细菌外毒素序列中特有模体,进一步理解外毒素的致病机制。【方法】构建非致病性细菌蛋白质数据库,利用InterProScan对数据库中非致病菌蛋白质序列以及收集的经实验确认的89条细菌外毒素蛋白质序列进行模体搜索。【结果】在89条细菌外毒素序列中,分析得到了39个细菌外毒素特有模体。【结论】得到的外毒素特有模体与外毒素功能密切相关,为在致病性细菌基因组内搜索外毒素序列奠定了基础;同时通过对外毒素特有模体的基因本体（Gene ontology,GO）注释分析,进一步阐明了细菌外毒素的致病机制。

[Objective]To identify the exotoxin-specific motifs/domains in bacterial exotoxin sequences,and to expand understanding of bacterial exotoxins pathogenic mechanisms.[Methods ] We constructed a non-pathogenic bacterial proteins database and collected 89 bacterial exotoxin sequences from Virulence factor database（VFDB）,then we analyzed these protein sequences by motif/domain search using InterProScan（www.ebi.ac.uk/Tools/InterProScan/）.[Results] We identified 39 exotoxin-specific motifs/domains in 89 bacterial exotoxin sequences.[Conclusion ] The identified exotoxin-specific motifs/domains were closely related to the functions of the exotoxins and could be used as template to search for new exotoxins by mining pathogenic bacterial genomes.The analysis of the acquired Gene Ontology（GO） items was to further expend our understanding of bacterial exotoxin pathogenic mechanisms.