摘要
目的:观察调脂积冲剂对非酒精性脂肪肝模型大鼠血清瘦素(LEP)和脂联素(APN)的影响。方法:45只雄性SD大鼠随机分为正常组10只、模型组12只、易善复组12只和调脂积组11只。每天上午,模型组、易善复组、调脂积组大鼠按1ml/100g体质量给予脂肪乳剂灌胃,正常组大鼠灌服等量蒸馏水。每天下午,按1ml/100g体质量给调脂积组大鼠灌服调脂积溶液,按1ml/100g体质量给易善复组大鼠灌服易善复溶液,模型组和正常组大鼠灌服等量生理盐水,均连续8周。观察各组大鼠肝指数、脂肪变性评分、炎症活动评分,ELISA法测定血清瘦素(LEP)和脂联素(APN),HE染色观察肝脏病理变化。结果:与正常组比较,模型组大鼠肝指数升高(P<0.01),脂肪变性及炎症活动评分均明显升高(P<0.01),LEP升高,APN降低(P均<0.01)。与模型组比较,调脂积组大鼠肝指数下降(P<0.05),脂肪变性及炎症活动评分均明显降低(P<0.01,P<0.05),LEP明显降低,APN升高(P<0.01,P<0.05);调脂积组各项指标与易善复组比较,差异无统计学意义(P>0.05)。结论:调脂积冲剂能明显改善肝组织细胞脂肪变性,缓解炎性细胞浸润,降低血清瘦素,升高脂联素水平,可能是其治疗脂肪肝的作用机制之一。
Objective：To observe the impact of the TiaozhiJi emulsion on the leptin （LEP） and adiponectin （APN） in fatty liver rats. Methods： Nonalcoholic fatty liver disease （NAFLD） model was established in rats. Rats were randomized divided into 4 groups ： normal control group （ N, n = 10 ）, model group （ M, n = 12 ） , Essentiale group （ E, n = 12 ）, and TiaozhiJi Emulsion group （T, n = 11 ）. Using enzyme - linked immunosorbent assay for determination of serum leptin and adiponectin levels. Liver pathological changes was detected by HE staining. Results：The increase in LEP and decline in APN in the M group were statistically significant as compared with the N group（P 〈 0.01 ）. Compared with the M group,the LEP level in the T group obviously droped, while the APN level was elevated （P 〈 0.05 ） ; TiaozhiJi emulsion significantly reduced the fatty degeneration in liver tissue cells（ P 〈 0.01, P 〈 0.05 ）, and ameliorated the inflammatory cell infiltration （P 〈 0.01 ,P 〈 0.05）. Efficacy of both treatment groups did not significantly differ （ P 〉 0.05 ）. Conclusion： TiaozhiJi emulsion can reduce the level of LEP and elevate the level of APN, as a result, the steatosis and inflammation in the liver ameliorate, which may be one of the mechanisms for the treatment of fatty liver.
出处
《浙江中西医结合杂志》
2010年第9期532-535,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省自然基金课题资助(No.Y2080541)
