摘要
目的探讨大鼠溃疡性结肠炎中JAK2的mRNA表达情况,初步阐明蛋白酪氨酸激酶JAK/信号传导子和转录激活子STAT信号通路在溃疡性结肠炎中调控的分子生物学机制。方法建立大鼠溃疡性结肠炎模型,提取结肠组织总RNA,经半定量RT-PCR检测JAK2 mRNA的表达情况。结果溃疡性结肠炎模型组JAK2的mRNA水平高于正常组,差异有统计学意义(P<0.05)。结论 JAK2在溃疡性结肠炎的发生、发展中起一定作用。
Objective To study the expression of JAKs mRNA in UC and explain the regulation mechanism of JAKs-STAT signal pathway in UC.Method Establish the model of ulcerative colitis of rats,isolate RNA from tissues,detect the mRNA expression of JAKs by RT-PCR.Result In rats ulcerative colitis model,the expression of mRNA of JAKs was higher than that in normal tissue.Conclusion JAK2 was related to the occurrence and development of UC.
出处
《新疆医科大学学报》
CAS
2010年第7期758-761,共4页
Journal of Xinjiang Medical University
基金
国家自然科学基金项目(30460163)
新疆医科大学大学生创新性实验计划项目(2008-5)
