谷氨酰胺对急性肺损伤大鼠核因子-κB及肿瘤坏死因子-α的影响

Effects of glutamine on NF-κB and TNF-α expressions in rats of acute lung injury

目的:探讨谷氨酰胺(GLN)对急性肺损伤核因子-κB(NF-κB)及肿瘤坏死因子-α(TNF-α)的影响。方法:健康清洁级SD大鼠32只,随机分为4组,每组8只。A组为生理盐水组,B组为油酸组,C组为油酸后0小时谷氨酰胺治疗组。D组为油酸后1小时谷氨酰胺治疗组。于注射油酸后4小时处死大鼠,免疫组织化学染色检测NF-κB、TNF-α在肺组织的表达并HE染色,观察肺组织变化。结果:在油酸诱导的急性肺损伤中,NF-κB和TNF-α表达上调,在油酸后0小时谷氨酰胺治疗组(组)NF-κB和TNF-α的表达明显低于油酸组(B组)(p<0.05),而油酸组与油酸后1小时谷氨酰胺治疗组(D组)无明显差异。结论:油酸性急性肺损伤时,肺组织NF-κB、TNF-α表达增强,谷氨酰胺早期给药可减轻这种影响,而减轻肺损伤。

Objective： To detect the protective effects of glutamine on NF-κB and TNF-α expressions in acute lung injury rats. Methods： The healthy Sprague - Dawley rats were randomly divided into 4 groups：control group（A）, oleic acid group（B）, glutanfine - treated group Oh after injected oleic acid（C） and glutamine-treated group lh after injected oleic acid. The animals were killed 4h after intravenous oleie acid. NF-κB and TNF -α of lung tissue was detected by immunohistochemistry, the data were analyzed with the SPSS 12.0 software, at the same time , lung tissue section were stained with hematoxylin and eosin, and observed change of lung tissue. Result ： Expressions of NF -κB and TNF -α in oleie acid - as- sociated acute lung injury were upregnlated. Compared with group B, the expressions of NF -κB and TNF-α in group C significantly decreased （ P 〈 0.05）. No significant difference was found between group B and D. Conlusion： The expressions of NF -κB and TNF -α may play an important role in the pathogenesis of oleie acid - associated acute lung injury in rats. Early administration of glutamine might decrease the effects and protect against oleic acid- associated acute lung injury.