植酸酶对日本沼虾生长、体组成和消化酶的影响

Effects of Dietary Phytase Supplementation on Growth Performance, Body Composition and Digestive Enzyme Activities in Oriental River Prawn Macrobrachium nipponense

在水温27±2.0℃的条件下,对体质量为0.199±0.089 g的日本沼虾进行了56 d的室内水循环系统饲养试验,探讨了添加植酸酶对日本沼虾生长、体组成和消化酶的影响。试验设7个组,其中试验组1-6分别在基础日粮中分别添加0、250、500、1 000、1 500和2 000 U/kg的植酸酶,试验组7不加植酸酶,加1.2%的磷酸二氢钙。每个试验组设3个重复。结果表明:添加植酸酶对日本沼虾成活率没有显著影响;WGR和SGR以添加500 U/kg植酸酶的试验组显著高于正对照组(P<0.05);植酸酶添加各组与对照组虾体的水分、粗蛋白、粗脂肪、粗灰分和总磷含量差异不显著;与正对照组相比,日本沼虾肝胰脏蛋白酶、脂肪酶活性提高显著(P<0.05)。结果说明,在特定试验条件下,饲料中添加植酸酶对日本沼虾的WGR、SGR有显著影响(P<0.05),对日本沼虾体组成影响不显著(P>0.05)。

A 56-day feeding trail was conducted to observe the influence of varying levels of phytase on growth performance,body composition and utilization of phosphorus in oriental river prawn Macrobrachium nipponense.The prawns（average wt.0.199±0.89 g） were stocked triplicately in a recirculating tank system（0.75 m×0.45 m×0.3 m） indoor.Seven trial diets were formulated for the experimental treatments.Exprimental group 1-6 were added different levels（0,250,500,1 000,1 500,2 000 U/kg） of phytase in the basic diet,respectively.The test group 7 was added 1.2% of the CaH2PO4,but without supplementation of phytase.The experimental shrimps were fed to satiation（twice/day） under the water temperature of 27±2.0 ℃.The results showed that there were no significant difference in survival rates among the different treatments with different phytase levels.WGR and SGR of the prawns fed the diets containing 500 U/kg phytase was significantly higher （P0.05） than those fed the diets containing 0 U/kg phytase;but no significant difference was found in whole body moisture,crude protein,crude lipid,ash and gross phosphorus levels among all treatments;compared with the control group,the activity of protease and lipase of hepatopancreas was increased significantly（P0.05）.