经济、有效提取外周血基因组DNA的方法

An economical and effective method of extracting peripheral blood genomic DNA

目的介绍一种经济、有效提取外周血基因组DNA的方法。方法采用0.2%NaCl处理人外周血并收集白细胞,再用基因组DNA提取试剂盒抽提基因组DNA。结果用原本提取100μL全血基因组DNA的试剂量来提取3mL全血的基因组DNA,费用节省了2倍以上。结论该方法方便、有效,同时还能得到大量的基因组DNA。

Objective To introduce an economical and effective method for extracting human peripheral blood genomic DNA.Methods The leukocytes were isolated from human peripheral blood by 0.2% NaCl and then the genomic DNA was extracted from the leukocytes with a DNA extract kit.Results The reagents volume of extract 100 μL peripheral blood genomic DNA by original method could extract 3mL peripheral blood genomic DNA by new method,with the cost being saved by more than two-fold.Conclusion This method is very economical and effective,meanwhile it can also obtain a large number of genomic DNA.