一种改良的纯化质粒DNA方法

An improved method of purification of plasmid DNA

目的介绍一种改良的纯化细菌质粒DNA的方法。方法针对传统的PEG纯化方法,取消PEG8000的纯化步骤,增加溶菌酶的浓度及酚-氯仿处理次数。结果新的纯化方法提取的质粒量比传统方法要多,而且纯度足以用于细胞研究。结论尽管目前已有质粒纯化试剂盒出售,但经改良的传统质粒纯化法仍然具有经济、实用的价值。

Objective To introduce an improved method of purification of bacterial plasmid DNA.Methods Based on the traditional method of PEG purification,the PEG8000 purification step was cancelled.However,the concentration of lysozyme and phenol-chloroform processing were increased.Results The content of extracting plasmid using the new purification method was more than the traditional method,and the purity was sufficient for cell research.Conclusion Although there are plasmid purification kits for sale,the improved method of purification of plasmid DNA also has a economic and practical value.