血浆与血清标本对心肌肌钙蛋白检测的影响

The influence of cardiac tropon I on the detection of plasma or serum specimen

目的探讨血浆与血清标本对心肌肌钙蛋白I(cTnI)检测的影响,以选用合适的标本缩短检测周转时间(TAT),优化实验规程。方法使用Beckman Coulter化学发光免疫分析仪分别检测74例患者血清、乙二胺四乙酸(EDTA)抗凝血浆、肝素抗凝血浆中cTnI水平。结果血浆与血清标本检测结果相关性良好,决定系数(r2)均为0.999。血清、EDTA抗凝血浆、肝素抗凝血浆阳性率分别为64.865%,60.811%,63.513%,三者之间比较差异无统计学意义。血清标本假阳性率为4.17%,肝素抗凝血浆假阳性率为2.13%,EDTA抗凝血浆假阴性率为3.45%。结论血浆标本对肌钙蛋白测定的影响较小。为缩短检测周转时间,优化实验规程,可考虑采用抗凝血浆代替血清。

Objective To explore the influence of cardiac tropon I （cTnI） on the detection of plasma or serum specimens,thereby to shorten the turnaround time （TAT） and optimize the experiment procedure by choosing a suitable specimen.Methods The levels of cTnI in serum and plasmas anticoagulated by EDTA or heparin were measured by Beckman Coulter chemiluminescence immue detection system.Results The testing data of serum was closely related with the testing datas of plasmas anticoagulated by EDTA or heparin.The coefficient of determinations （r2） were 0.999.Positive rates had no significant difference in serum （64.865%）,EDTA-plasma （60.8111%） and heparin-plasma （63.513%）.False positive rate of serum was 4.17%,false negative rate of EDTA-plasma was 3.45% and false positive rate of heparin-plasma was 3.45%.Conclusion The results demonstrated that the influence of cTnI on the detection of in plasma is less markable compared with serum.It is suggested that the plasmas anticoagulated by EDTA or heparin may replace the serum for shortening the TAT and optimizing the experiment procedure.