三种梅毒检测方法的应用评价

Evaluation of three methods for the detection of syphilis

目的探讨快速血浆反应素试验(RPR)、酶联免疫吸附试验(ELISA)、梅毒螺旋体明胶颗粒凝集试验(TPPA)三种方法对梅毒的诊断价值。方法分别采用RPR、TP-ELISA、TPPA三种方法对228例梅毒患者血清进行检测。结果 RPR法的灵敏度为73.0%,ELISA法为97.4%、TPPA为99.7%。RPR法与TP-ELISA法检测结果相比较差异有统计学意义(P<0.05),RPR法与TPPA法检测结果相比较差异有统计学意义(P<0.05),而ELISA与TPPA法检测结果相比较差异无统计学意义。结论梅毒检测方法各有优缺点,临床应根据检验目的的不同合理选用。

Objective To explore the value of rapid plasma reagin test （RPR）,enzyme-linked immunosorbent assay （ELISA） and treponema pallidum particle assay（TPPA）in the diagnosis of syphilis.Methods The serums of 228 syphilitic cases were detected by RPR,ELISA and TPPA.Results The sensitivity was 73.0 % for RPR,97.4% for ELISA,and 99.7 % for TPPA.The detection result of RPR had a significant difference compared with the detection results of ELISA and TPPA,while there was no significant difference between ELISA and TPPA.Conclusion Because each method of syphilic detection has merits and demerits,the selection of methed should be on the basis of check purpose.