摘要
目的研究Ⅰ型钙蛋白酶(calpain-I)在兔脑血管痉挛(CVS)模型中的表达,探讨钙蛋白酶在CVS中的意义。方法 25只健康清洁级新西兰大白兔,5只为正常对照组,20只采用血管内穿刺法制成脑血管痉挛模型,随机分为蛛网膜下腔出血(SAH)12h、1d、3d、7d组,用免疫组化染色(SP法)观察基底动脉平滑肌中calpain-Ⅰ的表达,并采用组织化学评分法进行半定量分析,观察其表达的变化。结果对照组基底动脉见极少量calpain-Ⅰ阳性染色平滑肌细胞,SAH12h组见少量阳性细胞,随着时间的延长,阳性细胞数显著增多,染色强度明显增加,以SAH后3d组为最明显,在SAH后7d组仍可见大量阳性染色平滑肌细胞,但染色强度下降。半定量分析显示SAH后3d为calpain-Ⅰ表达高峰,各组之间calpain-Ⅰ表达差异具有统计学意义(P<0.01)。结论 SAH后痉挛的脑血管平滑肌calpain-Ⅰ表达增强,且具有早期、渐变、持续性的特点,calpain-Ⅰ可能参与CVS血管壁结构性改变的信号调节过程。
Objective To investigate the expression of calpain-Ⅰ in rabbit model with cerebral vasospasm and to discuss the clinical significance of calpain-Ⅰ in cerebral vasospasm. Methods Twenty-five healthy New Zealand white rabbits were randomly divided into two groups：normal control group （n = 5） and subarachnoid hemorrhage （SAH） group （n = 20）. The subarachnoid hemorrhage was created by endovascular punctures. The SAH group,according to the time of sacrifice,was randomly and equally subdivided into 12-hour,1-day,3-day,and 7-day subgroups with 5 rabbits in each group. The expressions of calpain-Ⅰ of the smooth muscles of basilar arteries in both groups were determined by means of immunohistochemistry technique,and the semi-quantitative analysis of calpain-Ⅰ was evaluated by histochemistry score. Results The expression of calpain-Ⅰ was slightly elevated at 12 hours after SAH,it reached to its peak at the third day,and then declined on the seventh day. The semi-quantitative analysis of calpain-Ⅰ showed that the difference in the expression of calpain-Ⅰ between two groups was of statistic significance （P 〈0.01）. Conclusion After subarachnoid hemorrhage,the expression of calpain-Ⅰ in the spasmodic smooth muscles of basilar artery is enhanced,and the changes of the expression level is early,gradual and continuous. Most probably,the calpain-Ⅰ participates in the process of vessel wall structural signal transduction change in cerebral vasospasm.
出处
《介入放射学杂志》
CSCD
北大核心
2010年第8期631-634,共4页
Journal of Interventional Radiology
