摘要
目的建立RP-HPLC测定阿奇霉素缓释阴道栓的含量。方法用C18(4.6 mm×150 mm,5μm)柱,以乙腈-磷酸氢二钾溶液(0.02 mol/L)-磷酸二氢钾溶液(0.07 mol/L)溶液(60:25:15)为流动相,柱温40℃,流速1.2 mL/min,检测波长215 nm。结果阿奇霉素的线性范围为60～800μg/mL,r=0.999 8;低、中、高3种浓度的平均回收率分别为101.4%,99.1%,101.2%。结论本方法简便、灵敏、准确。
Objective To establish a RP-HPLC method for the determination of azithromycin in azithromycin sustained-release vaginal suppository.Methods A C-18 column（4.6 mm×150 mm,5 μm）was used with acetonitrile-0.02 mol·L-1 dipotassium hydrogen phosphate-0.07 mol·L-1 monobasic potassium phosphate（60：25：15）as the mobile phase.The column temperature was 40 ℃.The flow rate was 1.2 mL？min-1 and the detection wavelength was 215 nm.Results The linear range of azithromycin was 60 μg？mL-1～800 μg？mL-1（r=0.999 8）.The average recoveries of low,medium and high concentration were 101.39 %,99.07 %,100.23 % and RSDs were 0.80 %,0.58 %,0.93 %（n=6）,respectively.Conclusion The method is simple,sensitive and accurate.
出处
《食品与药品》
CAS
2010年第9期328-330,共3页
Food and Drug
基金
烟台市科学技术发展计划项目(2008602)
