青蒿水提物对小鼠生殖功能及胎鼠生殖发育的影响

Toxic Effects of Artemisia Aqueous Extract on General Reproduction in Mice

目的:观察青蒿水提物对雌性小鼠生殖功能及胚胎发育的影响。方法:80只健康昆明种雌小鼠随机分成4组,实验组连续14 d分别腹腔给予青蒿水提物20 mg/kg(低剂量组)、100 mg/kg(中剂量组)和500 mg/kg(高剂量组),对照组以等量生理盐水代替,然后与雄鼠合笼,同组内一半雌鼠继续给药至交配成功后14 d处死;另一半雌鼠在受精后继续给药35 d,自然分娩,检测青蒿水提物对各组母鼠生育能力和胎仔生长发育的影响。结果:高剂量组青蒿水提物可以使孕鼠的胎仔数以及仔鼠平均体质量、胎盘质量下降,吸收胚胎数增加;但对雌鼠的交配指数、生育指数、活产指数及对出生仔鼠的存活指数、生长指数均无明显影响(P>0.05)。结论:青蒿水提物对孕鼠胚胎有影响,但对雌鼠的其它生殖功能无明显毒性作用。

Objective：To observe the toxic effects of artemisia aqueous extract on the ability of maturity,mating,fertility and early growth and development.Methods： A total of 80 Kunming female mice were randomly divided into 0 mg/kg（the control）,20 mg/kg（low-dose group）,100 mg/kg（mid-dose group） and 500 mg/kg（high-dose group） groups,and artemisia aqueous extract was administered intraperitoneally for 14 d in female mice before mated.The artemisia aqueous extract treatment continued until 14th day of pregnancy and 35th day of fertilization.Since the childbirth,the influence of artemisia aqueous extract on the ability of reproduction and fetal growth and development was detected.Results： Decrease of the number of live embryo and the average weight of placenta,fetal mice and increase of absorbed embryo in high-dose group were observed（P0.05）.There were no significant differences in the index of mating,fertility of female and live birth,early fetal growth and development between the treatment groups and the control.Conclusion： Artemisia aqueous extract can obvi-ously impact embryonic and fetal development but did not affect other reproduction functions of mice.