卵泡液中TNF-α和IFN-γ与卵丘颗粒细胞凋亡关系的探讨

Correlation between TNF-α and IFN-γ and the Apoptosis of Granulosa Cells in Human Follicular Fluid

目的:探讨卵泡液中肿瘤坏死因子α(TNF-α)和γ干扰素(IFN-γ)与颗粒细胞凋亡的关系。方法:应用TUNEL方法测定卵丘颗粒细胞凋亡率,应用酶联免疫方法(ELISA)测定成熟卵泡液中TNF-α和IFN-γ的浓度,探讨其相关性。结果:妊娠组颗粒细胞凋亡率为12.25±4.84%,明显低于未妊娠组的15.96±7.04%(P<0.05);TNF-α浓度与颗粒细胞凋亡率的相关系数r=0.56,P<0.05,两者存在显著的正相关关系;IFN-γ浓度与颗粒细胞凋亡率的相关系数r=-0.57,P>0.05,两者不存在相关关系。结论:卵泡液中TNF-α浓度与颗粒细胞凋亡呈正相关,IFN-γ浓度与颗粒细胞凋亡无相关关系,颗粒细胞凋亡率高的患者不易获得妊娠。

Objective：To investigate the relationship between the concentration of tumour necrosis factor-α（TNF-α） and interferon-gamma（IFN-γ） in follicular fluid and granulosa cell apoptosis in IVF-ET treatment.Methods： TUNEL was used to analyze granulosa cell apoptosis.Enzyme linked immunosorbent assay（ELISA） wasused to analyze the concentrations of TNF-α and IFN-γ in mature follicular fluid.The IVF treatment outcome was closely followed up.The relationship between the concentration and granulosa cell apoptosis rate was discussed by statistical analysis.Results： The apoptosis rate of the granulosa cells（15.96±7.04%） in the patients without successful IVF-ET outcome was significantly higher than that in pregnant group（12.25±4.84%）,P0.05.Correlation coefficient（r） of the TNF-α and the granulosa cells apoptosis rate was 0.56,which had a statistical significance（P0.05）,while the correlation coefficient of the IFN-γ and the granulosa cell apoptosis rate was-0.57,which had no statistical significance（P0.05）.Conclusion： Higher concentration of TNF-α will cause higher granulosa cell apoptosis rate,while IFN-γ has no effect on the granulosa cells apoptosis.Women received IVF treatment with higher granulosa cells apoptosis rate in mature follicular fluid is more difficult to get pregnancy.