microRNA在先天性心脏病继发性肺动脉高压肺组织内变化的实验研究

Analysis of microRNA Expression Patterns in Pulmonary Arterial Hypertension Associated with Congenital Heart Disease

近年来发现多种miRNA在心血管系统中发挥了极其重要的作用。应用microRNA(miRNA)芯片技术对miRNA在先天性心脏病相关肺动脉高压(pulmonary arterial hypertension,PAH)肺组织内的变化进行研究。收集室间隔缺损(ventricular septal defect,VSD)合并重度PAH(PAH组,n=6)和不伴PAH患者肺组织(对照组,n=6)。分别提取总RNA,然后采用miRNA芯片进行miRNA表达谱差异分析,并对结果进行实时定量PCR验证。和对照组相比,PAH组miRNA表达谱中共有78个差异表达位点,其中有62个上调,16个下调。提示miRNA参与了先天性心脏病继发性肺动脉高压的调节作用,可能成为预防和逆转PAH的新靶点。

It has been found that several miRNAs play a great role in cardiovascular system recently. To investigate the expression pattern of microRNA （miRNA） in pulmonary arterial hypertension （PAH） associated with congenital heart disease （CHD） by miRNA array, lung tissues were collected from patients with severe PAH due to ventricular septal defect （VSD） （PAH group, n=6） and patients with VSD but without PAH （control group, n=6）. Total RNA was extracted from tissues collected and the different expression of miRNA was detected by miRNA array and also verified using real-time quantitative reverse transcriptase-polymerase chain reaction （qRT-PCR）. Among 1 468 target miRNAs, 78 miRNAs showed different expression levels, 62 miRNAs were up-regulated and 16 miRNAs were down-regulated. In conclusion, miRNA may become a new target of PAH reversing and preventing drug since it is involved in the development of PAH associated with CHD.