摘要
本文介绍用稳定同位素^(13)C标记的辛酸混入液体和固体试餐喂饲小鼠,通过检测小鼠餐后3小时内设定时间点呼气中^(13)CO_2的丰度,根据胃动力计算公式,用数据拟合出小鼠胃排空曲线,计算出小鼠胃排空三个主要参数:GEC、t_(1/2)、t_(lag),检测小鼠胃动力的功能。
Gastric emptying studies in the laboratory mouse are hampered for the lack of a non-invasive and repeatable technique. The aim of the present study was to adapt the non-invasive [^13C]-octanoic acid breath test to assess gastric emptying of both liquid and solid meal in the mouse. Gastric emptying rates were investigated for a liquid meal （Intralipid） and a solid meal （egg yolk） incorporating [^13C]-octanoic acid. Mathematical analysis of the ^13CO2 excretion rate allowed the calculation of gastric emptying parameters （GEC, t1/2, and qag）- The breath test can continuously and non-invasively assess gastric emptying in the mouse. This method can be used to assess gastric emptying in drug development and disease studies in the mouse.
出处
《中国细胞生物学学报》
CAS
CSCD
2010年第4期621-625,共5页
Chinese Journal of Cell Biology
基金
国家国家高技术研究发展计划(863计划)(No.2008AA02Z126)资助项目~~
关键词
胃排空
胃动力
^13C-辛酸
呼气试验
小鼠模型
gastric emptying
gastric motility
[^13C]-octanoic acid
breath test
mouse model
