山莨菪碱对大鼠缺血后肢侧支循环建立的作用

Effect of Anisodamine in Collateral Circulation of Ischemic Hind Limb of Rat

目的探讨山莨菪碱对大鼠缺血后肢侧支循环建立的影响。方法建立左后肢缺血模型后,随机分为治疗组和对照组,分别给予山莨菪碱注射液8 mg/(kg.d)和等量生理盐水,于缺血后肢分5点进行肌内注射,连续14 d;观察术后即刻、7、14、28 d健侧后肢与缺血后肢脚垫温差及术后28 d大鼠后肢肌肉组织中的微血管密度(Microvessel density,MVD)。结果治疗组两侧后肢脚垫温差(1.75±0.18)℃,较对照组(2.57±0.13)℃小(P<0.05);治疗组缺血后肢肌肉组织中的MVD(4.60±1.50)较对照组(3.33±1.05)高(P<0.05)。结论山莨菪碱对大鼠缺血后肢侧支循环的建立有促进的作用。

Objective To study the effect of anisodamine for the SD rat ischemic hind limb collateral circulation.Methods To establish the left hind limb ischemia model and to divide the SD rats into treatment group（15 cases） and control group（15 cases）.The treatment group was treated with anisodamine 8 mg /（kg·d）,and Sub-5 places in the ischemic lower limb for intramuscular injection consecutive 14-day.As well as the control group was treated with equivalent of 0.9% sodium chloride injection,and Sub-5 places in the ischemic lower limb for intramuscular injection consecutive 14-day.Hind limbs in modeling observed immediately after surgery,7 days after surgery,14 days after surgery,28 days after surgery at different time points,changes in skin temperature and 28 days after ischemic hindlimb muscle tissue of the microvessel density.Results The treatment group（1.75±0.18） ℃,both sides of the hind foot pad temperature were smaller than the control group（2.57±0.13） ℃（P0.05）;The treatment group in the muscle tissue of ischemic hind limb than the control group were a higher MVD（P0.05）.Conclusion Anisodamine on ischemic hind limb collateral circulation can promote the role of the establishment.