FcRL3基因单核苷酸多态性与中国汉族强直性脊柱炎相关性研究

Fc receptor like 3 in Han nationality of China patients with ankylosing spondylitis

目的 探讨Fc受体样基因3（FcRL3）部分单核苷酸多态性（SNP）在中国汉族强直性脊柱炎（AS）发病中的作用.方法 选取AS患者160例,健康对照组170例.用Mass-ArrayTM SNP检测仪对FcRL3基因上的SNP位点：FcRL3-1（rs0158440）、FcRL3-2（rs2225828）、FcRL3-3（rs7528684）、FcRL3-4（rs11284799）、FcRL3-5（rs945635）、FcRL3-6（rs3761959）、FcRL3-7（rs2210913）、FcRL3-8（rs2282284）、FcRL3-9（rs2282283）SNP进行检测.单倍型构建采用PHASEv2.1软件完成.结果 在AS组中FcRL3-1-1651C、FcRL3-3-169C、FcRL3-6 Intron3A、FcRL3-7 Intron3A等位基因频率病例组高于对照组 FcRL3-2-641C、FcRI3-4-110A、FcRL3-9 Intron15A等位基因频率病例组低于对照组 FcRL3-6、FcRL3-5和FcRL3-8SNP等位基因两组比较差异无统计学意义.AS组中单倍型FcRL3-1→9 CTCGCAAAA、CCCGGGGAA、CCCGCAAAC、CTCGCAAAC频率明显高于对照组 FcRL3-1→9 CCTGGGGAA和TCCACAAGA频率明显低于对照组.结论 FcRL3基因与中国汉族AS的易感性相关.

Objective To investigate the association between a few single nucleotide polymorphisms（SNPs） in Fc receptor like 3 and ankylosing spondylitis（AS） in Hah nationality of China patients.Methods One hundred and sixty AS patients along with one hundred and seventy healthy controls were included in the study. SNPs of FcRL3-1 （rs0158440）, FcRL3-2 （ rs2225828 ）, FcRL3-3 （ rs7528684 ）,FcRL3-4（rs11284799）, FcRL3-5（rs945635）, FcRL3-6（rs3761959）, FcRL3-7 （rs2210913）, FcRL3-8 （ra2282284） and FcRL3-9 （rs2282283） in FcRL3 gene were genotyped by MALDI-TOF technology. Haplotypes were estimated using PHASE v2.1 software. Results The frequency of FcRL3-1-1651C, FcRL3-3-169C, FcRL3-6 Intron3A and FcRL3-7 Intron3A alleles in AS patients were significantly increased and the frequency of FcRL3-2-641C, FcR-L3-4-110A and FcRL3-9 Intron15A were significantly increased compared with healthy controls. The frequency of FcRL3-1→9 CTCGCAAAA, CCCGGGGAA, CCCGCAAAC and CTCGCAAAC haplotypes were significantly increased and the frequency of FcRL3-1→9CCTGGGAA and TCCACAAGA haplotypes were significantly decreased compared with healthy controls. Conclusion These results suggest that FcRL3 may be associated with AS in Han nationality of China.