胃肠道肿瘤患者外周血人类斯钙素基因的检测及其意义

Clinical significance of detection of stanniocalcin 1 expression in peripheral blood of patients with gastrointestinal cancer

目的:探讨胃肠道恶性肿瘤患者外周血人类斯钙素基因表达及其与肿瘤微转移的关系.方法:采用RT-PCR方法检测胃肠道恶性肿瘤患者40例,消化系炎症性疾病患者10例和正常健康捐赠者10例的外周血,以及患者6例(胃癌2例、大肠癌4例),同时于术中取肿瘤组织及正常肠道组织的hSTC-1mRNA.结果:在胃肠道恶性肿瘤患者40例中,外周血hSTC-1 mRNA的表达的阳性率为60%(24/40例),肿瘤组织6例均为阳性,癌旁正常组织仅1例为阳性,其余健康成人10例及消化系炎症性疾病患者10例均为阴性.结论:在胃肠道肿瘤患者的外周血中检测hSTC-1 mRNA的表达是判定肿瘤早期微转移的一种较好的方法,他可以作为肿瘤转移和复发以及疗效观察的客观指标之一.

AIM：To detect the expression of stanniocalcin 1 mRNA（STC1） in peripheral blood of patients with gastrointestinal cancer and to analyze its relationship with tumor micrometastasis.METHODS：RT-PCR was performed to detect the expression of STC1 mRNA in peripheral blood samples obtained from 40 patients with gastrointestinal cancer,10 patients with gastrointestinal inflammatory disease and 10 healthy blood donors,and in tumor tissue and tumoradjacent normal intestinal tissue samples taken from 2 patients with gastric cancer and 6 patients with colorectal cancer.RESULTS：The positive rate of STC1 mRNA expression was 60%（24/40） in peripheral blood from patients with gastrointestinal cancer,100%（6/6） in tumor tissue and 16.7%（1/6） in tumoradjacent normal intestinal tissue from patients with gastric cancer or colorectal cancer.STC1 mRNA expression was undetectable in peripheral blood from patients with gastrointestinal inflammatory disease or healthy blood donors.CONCLUSION：Detection of STC1 mRNA expression in peripheral blood can be used to predict early micrometastasis and therefore represents an objective indicator for evaluation of tumor metastasis and recurrence and therapeutic efficacy in patients with gastrointestinal cancer.