内皮素对心血管系统内血管紧张素Ⅱ释放的影响

EFFECT OF ENDOTHELIN ON THE RELEASE OF ANGIOTENSIN I FROM RAT CARDIOVASCULAR SYSTEM

采用放免测定及免疫组化技术证明大鼠心血管系统内有血管紧张素Ⅱ免疫活性物质的存在,其心房、主动脉组织及培养的主动脉平滑肌细胞内的含量分别为:7.2±2.7pg/mg 蛋白、152±59.2pg/mg 蛋白和3.5±0.8pg/2×10~5细胞。内皮素是由内皮细胞产生的一个具有强大缩血管功能的多肽,可显著增强培养的鼠主动脉及鼠主动脉细胞释放血管紧张素Ⅱ。这一结果表明,很可能存在一个区域化的内皮素——血管紧张素相互作用,这个相互作用可能参与局部血流及血管紧张度的调节,也还可能参与心肌肥厚,血管肥厚等疾病过程。

The existence of angiotensin Ⅱ immunoreactive substance in rat cardiovascularsystem was demonstrated by RIA and immunohistochemistry techniques.In atrium,aorta and cultured rat aorta smooth muscle cells,angiotensin Ⅱcontent is 7.2+2.7pg/mg protein,152.4+59.2 pg/mg protein and 3.5+0.8pg/2×10~5 cells respectively.Endothelin,a potent vasoconstricting polypeptide,can enhance the release ofangiotensin Ⅱ from cultured rat aorta and aortic smooth muscle cells significantly.The results suggest that endothelin may be involved in the regulation of local bloodflow and vascular tone,and it may also implicate the pathogenesis of some cardiovas-cular diseases such as cardiohypertrophy and vascular hypertrophy.