电刺激促进成骨细胞在导电可生物降解材料上活性的研究

Electrical Stimulation Enhances Viability of Osteoblasts on Conductive Biodegradable Material

探求种植在导电组织工程材料上成骨细胞的活性。导电组织工程材料聚吡咯/聚乳酸(PPy/PLA)复合膜由乳液聚合法制得。扫描电子显微镜和红外光谱检测材料显示,PPy均匀地分散在PLA基质中,直流电刺激系统测试得到复合膜可以维持一个月以上导电性稳定性,为细胞生长提供电环境。扫描电镜显示原代培养的成骨细胞能在PPy/PLA复合膜上很好的黏附和伸展。使用12.5、25、50、75、100、125、150、175、200μA/cm2电刺激成骨细胞24、48、72和96 h后,分别通过四甲基偶氮唑(MTT)比色法、碱性磷酸酶(ALP)活性、无机钙测定得到,24 h后50μA/cm2明显促进细胞增殖;48 h后50μA/cm2和75μA/cm2与对照组相比显著促进细胞分化;同时促进细胞矿化。最终得出,50μA/cm2的电刺激加强成骨细胞功能,促进细胞活性更有积极的作用。

This study was aimed at exploring the viability of osteoblasts on conductive tissue engineering material.Conductive biodegradable Polyprrole/Polylactide（PPy/PLA） was prepared by emulsion polymerization.Scanning electron microscope（SEM） and spectroscopy showed evenly dispersed PPy in PLA.PPy/PLA membrane was found being able to keep conductive stability for more than one month to provide electric circumstances（ECs） for osteoblasts.SEM displayed that osteoblasts adhered and spread well on PPy/PLA.ECs of 12.5,25,50,75,100,125,150,175,200μA/cm2 were separately used to stimulate osteoblasts for 24h,48h,72h and 96h.Methyl-thiazole-tetrazolium（MTT） assay after 24h revealed that 50μA/cm2 evidently accelerated osteoblasts proliferation.Alkaline phosphatase（ALP） activity assay revealed that,48h later,50μA/cm2,and 75μA/cm2 promoted osteoblasts differentiation.50μA/cm2 enhanced osteoblasts mineration.Conclusively,50μA/cm2 can strengthen osteoblasts＇ function and promote their viability.