组织工程胶原海绵支架材料的制备及孔径结构分析

The Preparation of Collagen Sponge as Tissue Engineering Scaffolds and Analysis of Its Pore Structure

制备组织工程胶原海绵支架材料,采用聚乙二醇脱水法将胶原溶液于4℃下进行分时段脱水,制得不同胶原浓度的溶液,并通过真空冷冻干燥法,制成多孔海绵材料。利用激光共聚焦显微镜、扫描电镜、万能拉力机等手段对海绵材料的结构及性能进行分析表征。统计结果表明,不同胶原浓度的溶液制备出不同孔径、孔隙率及平衡含水率的海绵材料,且力学性能也存在差异。从而通过控制胶原溶液的浓度,可制备不同孔径大小的海绵,以适应构建不同种类的组织工程支架材料的需要。

The preparation of collagen sponges was studied in order to develop tissue engineering scaffolds.Collagen solutions with varying concentrations were obtained by condensing the initial collagen with polyethylene glycol（PEG） at 4℃ for different periods of time,and then were freeze-dried to make collagen scaffolds.The porous characteristics of the prepared scaffolds were characterized by use of different methods,including laser scanning confocal microscopy（LSCM）,scanning electron microscopy（SEM） and tensile tests.All collagen sponges were shown to have similar interconnected porous structures but were found to have different pore size,porosity,water capacity and the mechanical property,depending on the concentration of collagen solutions.These findings indicate that the way of controlling the concentration of collagen solutions with PEG permits the freeze-drying fabrication of collagen sponges with varying porous features suitable for different tissue engineering purposes.