不同浓度纳米银对人脐静脉内皮细胞与人脐动脉平滑肌细胞增殖性能影响的研究

Influence of the Concentration of Silver Nanoparticles on the Proliferation Behavior of Human Umbilical Vein Endothelial Cell And Human Umbilical Artery Smooth Muscle Cells

利用MTT、BCA和流式细胞仪等方法研究不同浓度的纳米银对人脐静脉内皮细胞(HUVEC)和人脐动脉平滑肌细胞(HUASMC)的增殖性能的差异性影响,对实验结果进行了方差统计分析,并对造成的差异性影响原因进行了初步讨论。实验结果表明,两种细胞对纳米银浓度的增殖响应有显著差异,这种差异来自于纳米银浓度和培养基中血清含量不同引起的两种细胞间细胞形态和凋亡率之间的差异。

The proliferation difference between Human Umbilical Vein Endothelial Cells（HUVEC） and Human Umbilical Artery Smooth Muscle Cells（HUASMC） in response to the concentration of silver nanoparticles was investigated via MTT、BCA and FCM tests.The obtained experimental data were statistically analyzed and discussed in order to know the causation of the proliferation difference.The results show there is significant difference in proliferation between HUVEC and HUASMC corresponding to the concentration of silver nanoparticles,and such difference can be attributed to the varied adhesion shape and apoptosis of the cells being influenced by nano-Ag content and Fetal bovine serum（FBS） content in culture medium.