摘要
目的观察高脂饮食对老年大鼠骨骼肌肉碱棕榈酰基转移酶Ⅰ(CPTⅠ)和过氧化体增殖物激活受体γ(PPARγ)表达的影响及罗格列酮的干预效果。方法大鼠随机分为对照组、高脂组(HF)、罗格列酮干预组(RSG),每组20只。应用清醒状态下正常葡萄糖高胰岛素钳夹技术的葡萄糖输注率评价胰岛素抵抗,用RT-PCR法和Western blot印迹技术检测骨骼肌CPTⅠ和PPARγ的表达。结果高脂组骨骼肌三酰甘油(TG)升高而葡萄糖输注率明显下降(P<0.01),骨骼肌CPTⅠ和PPARγ表达明显降低(CPTⅠbmRNA 3.16±0.59vs1.30±0.18,P<0.05;PPARγmRNA1.48±0.25vs1.04±0.33,P<0.01),罗格列酮干预组显著缓解高脂组上述变化(CPTⅠbmRNA1.30±0.18vs2.84±0.72;PPARγmRNA1.04±0.33vs2.13±0.42)(P<0.01)。结论罗格列酮上调高脂饮食老年大鼠骨骼肌CPTⅠ和PPARγ的表达,可能是减少骨骼肌内脂质堆积、改善胰岛素抵抗的机制之一。
Objective To observe the expression of carnitine palmitoyltransferase Ⅰ（CPTⅠ） and peroxisome proliferators activated receptorγ（PPARγ） in high-fat-fed and aged rats and rosiglitazone intervention.MethodsWistar rats were divided into control group,high-fat diet（HF） group and high-fat diet plus rosiglitazone maleate tablets（RSG） group,（n=20 in each group）.Insulin sensitivity was evaluated by conscious hyperinsulinemic-euglycemic clamp techniques,the expression of CPTⅠ mRNA and PPARγ mRNA and protein were analyzed by RT-PCR and Western blot.Results Skeletal muscle triglyceride was significantly higher and glucose infusion rate was significantly lower in the control group than those in the high-fat diets group.The expression of CPTⅠ and PPARγ decreased in HF group,RSG group reserves above of all the changes.Conclusion An increased expression of CPTⅠand PPARγ play a pivotal role in insulin-sensitizing effect of rosiglitazone treatment,and this could be one of the mechanisms of insulin resistance.
出处
《基础医学与临床》
CSCD
北大核心
2010年第9期935-939,共5页
Basic and Clinical Medicine
基金
河北省自然科学基金(C2006000891)
