糖尿病大鼠外周神经山梨醇通路和 Na^+—K^+—ATP 酶活性的变化

ALRATIONS IN THE SORBITOL PATHWAY AND Na_+-K_+-ATPase ACTIVITY OF PERIPHERAL NERVE OF ALLOXAN-INDUCED DLABETIC RATS

本文用四氧嘧啶诱导产生糖尿病动物模型,分别于糖尿病产生后第4,8,12周测定大鼠坐骨神经匀浆山梨醇通路活性,肌醇含量和哇巴因敏感的和不敏感的 ATP 酶活性。与同龄正常对照组比较,糖尿病发生4周后,坐骨神经葡萄糖含量增加3—4倍,果糖增加3—5倍,山梨醇增加6—9倍,肌醇含量降低到对照组的50%,总 ATP 酶和哇巴因敏感的Na^+-K^+-ATP 酶活性均极显著地低于同龄对照组(P<0.01)。结果提示这些代谢变化可能是糖尿病神经病变发病机制中的重要环节。

Sorbitol pathway activity,myo-inositol content and ouabain-sensitive andouabain-resistant ATPase activities were measured in homogenates of the sciaticnerve from alloxan-induced diabetic rats for 4,8 and 12 weeks.Compared with theage-matched control,glucose,fructose and sorbitol contents of the sciatic nervefrom diabetic rats for 4,8 and 12 weeks were increased respectively to a level of3-4 fold,3-5 fold and 6-9 fold of those of the control rats.Myo-inositol wasreduced approximately 50%.There was a significant decrease(P<0.01)in totalATPase,ouabain-sensitive and ouabain-resistant ATPase activities.These changescould be relevant to the development of diabetic neuropathy.