NaCl对高蛋白糯稻品系H1701胚培养力的影响

The effect of NaCl on plant regeneration from embryo culture of rice (Oryza sativa L. )

以糯稻H1701为材料，通过胚培养诱导愈伤组织，继代培养并分化．实验结果表明；NaCl对胚性愈伤组织的诱导和生长有不同程度的抑制作用．NaCl浓度越高，抑制越明显．转入分化培养基以后，除含10g／L及以上浓度NaCl的诱导培养基中愈伤组织分化为零外，其余均能分化出苗．其中．含NaCl2g／L的诱导培养基中诱导出的愈伤组织分化率最高．

In the present report, the effect of NaCl on plant regeneration from ma-ture embryo culture of rice (Oryza sativa L )in vitro was studied. The concentra-tlon of NaCl below 6. O g/L, in induction medium and proliferation medium reducedcallus growth slightly, but did not decrase regeneration frequency. Exposure toNaCl above 6. 0 g/L, during induction and proliferation phage decreased the rates ofinduction and regeneration.