摘要
目的 通过观察帕立骨化醇（paricalcitol）对血管紧张素Ⅱ（AngⅡ）诱导的成纤维细胞的转分化的干预作用,旨在探讨帕立骨化醇抗肾间质纤维化的潜在效应及相关机制.方法 体外培养大鼠肾间质成纤维细胞（NRK-49F）,分别以终浓度为10^-6mol/L AngⅡ和（或）终浓度为10^-6mol/L的帕里骨化醇进行干扰.应用免疫细胞化学染色法检测α-肌动蛋白（α-SMA）的表达.结果 帕立骨化醇（10^-6mol/L）能明显抑制正常和AngⅡ（10^-6mol/L）诱导的NRK-49F α-SMA的表达,并在12至48小时范围内呈时间依赖性显著降低AngⅡ诱导的α-SMA蛋白表达（p＜0.05）.结论 帕立骨化醇具抗肾间质成纤维细胞转分化的潜在作用.
Objectives To observe the effects of paricalcitol on the effects of the transdifferentiation of the renal interstitial fibroblasts that induced by angiotensin Ⅱ ,so as to illustrate its potential roles and underlying mechanisms in preventing renal interstitial fibrosis. Methods In NRK-49F of cultured rats in vitro, the expression of α-SSA was dected by immunocytochemistry. Results Increased expression of α-SMA induced by angiotensin Ⅱwas significantly suppressed by paricalcitol as compared with angiotensin Ⅱ treated group （p 〈 0.05）. Conclusions There was difference among the groups with various concentration of paricalcitol.
出处
《国际泌尿系统杂志》
2010年第5期616-618,共3页
International Journal of Urology and Nephrology
