大鼠神经源性肺水肿时交感神经放电活动、血浆儿茶酚胺含量及肺表面活性物质的改变

ALTERATIONS OF THE SYMPATHETIC ACTIVIIY,PLASMA CATECHOLAMINE CONTENT AND PULMONARY SURFACTANT IN NEUROGENIC PULMONARY EDEMA IN RATS

红藻氨酸(kainic acid,KA)注入大鼠双侧下丘脑外侧视前区(POA)复制神经源性肺水肿(NPE),观察左侧交感神经肾上腺支放电活动,测定血浆儿茶酚胺含量及肺泡灌洗液中肺表面活性物质(pulmonary surfactant,PS)物理特性变化。大鼠 POA 注入 KA 后交感神经放电频率迅速增加,(?)O和60min 时分别增加22.8±7.2%和32.2±8.0%。血浆儿茶酚胺含量增高,在30和60min 时最明显。PS 物理特性也有变化,最大表面张力降低,最小表面张力升高,回弹系数及稳定系数均下降,迟滞环面积缩小。实验结果表明:早期即有 PS 活性变化,交感神经神经放电活动和血浆儿茶酚胺含量变化在时间上相平行。这种神经源性肺水肿的发生可能与下丘脑水平自主神经功能紊乱、交感神经发放增加、血浆儿茶酚胺增多有关,PS 表面活性下降也有一定作用。

Neurogenic pulmonary edema(NPE)was induced by microinjection of kainicacid into bilateral preoptic area(POA)of the hypothalamus in rats.Sympatheticdischarge of the left adrenal branch was recorded,plasma catecholamine(CA)wasa ssayed and the physical properties of pulmonary surfactant(PS)of the lung lavagewere measured,The results showed that some physical properties of PS were changedin NPE,i.e.maximal surface tension(γ Max)decreased,minimum surface tension(γ Min)increased,recruitment index(RI),stability index and area of hysteresisloop(H-area)decreased.After injection of kainic acid into POA,the sympatheticdischarge increased by 22.8±7.20% and 32.2±8.0% respectively at 30 and 60 minafter injection and paralleled by a marked elevation of plasma catecholamine(CA).The results showed that PS activity had decreased at an early stage of NPE and thechange of the plasma CA level was parallel to that of sympathetic discharge.It issuggested that generation of NPE may be related to some disorder of the autocomicnervous system at the level of hypothalamus,increase of sympathetic discharge,elevation of plasma CA level and fall of PS activity.