电针大鼠的血清中淋巴细胞转化抑制因子的作用机制分析

POSSIBLE MECHANISMS OF THE ACTION OF LYMPHOCYTE PROLIFERATION-INHIBITORY FACTOR(S)IN RAT SERUM RECEIVING ELECTROACUPUNCTURE STIMULATION

本室以前的工作表明:电针(2H_z,3V,30min/d)刺激 SD 大鼠双侧足三里-三阴交,5d后,大鼠血清中产生出淋巴细胞转化抑制因子,本工作对此抑制因子的作用机制进行了初步研究,主要结果如下:(1)电针大鼠的血清不仅显著抑制 Con A 刺激的小鼠淋巴结 T 淋巴细胞转化,还可显著抑制 Con A 刺激的小鼠胸腺细胞和脾脏 T 淋巴细胞转化;同时也发现电针大鼠的血清能显著抑制脂多糖(LPS)刺激的小鼠淋巴结 B 淋巴细胞转化。提示此淋巴细胞转化抑制因子对不同淋巴器官及不同类型的淋巴细胞无选择性作用。(2)将电针大鼠的血清同小鼠淋巴结细胞培养1h,电针大鼠的血清就可显著抑制 Con A 刺激的 T 淋巴细胞转化;将小鼠淋巴结细胞同 Con A 预培养30min,电针大鼠的血清的抑制作用便消失,提示电针大鼠血清中淋巴细胞转化抑制因子作用于 Con A 刺激 T 淋巴细胞活化的早期阶段,同时也排除了此抑制因子的细胞毒作用。(3)电针大鼠的血清显著抑制蛋白激酶 C(PKC)激活剂 PMA和 PMA 加 ca^(2+)通道 A23187刺激的小鼠淋巴结细胞转化,提示淋巴细胞转化抑制因子通过抑制 PKC 的活性或抑制 PKC 介导的细胞活化通路,抑制有丝分裂原刺激的淋巴细胞转化。

Previous reports showed that EA stimulation(3V,2Hz,30 min/d,5 d)in-duced the production of one or more lymphocyte proliferation-inhibitory factor(s)inthe rat serum.In this paper,the mechanisms of the action for the inhibitory factor(s)to suppress lymphocyte proliferation were studied.(1)the lymphocytes from dif-ferent immune organs of the mice were prepared and cultured with the rat serumstimulated by EA.The results show that the serum not only inhibited the mouselymphonodus T cell proliferation induced by Con A,but also inhibited the mousethymocyte and spleen T cell proliferation induced by Con A.When B cells werestimulated by LPS,the proliferative effect can also be inhibited significantly bythe rat serum stimulated by EA.This implies that the effect of the lymphocyteproliferation-inhibitory factor(s)has no specificity.(2)Incubation of the mouselymphonodus cell with serum for one hour is enough to cause an inhibitory effect onCon A stimulated lymphocyte proliferation.However,no inhibitory effect wasobserved if the mouse lymphonodus cells were incubated with Con A for 15min or3O rain before the addition of rat serum.The results demonstrate that the lympho-cyte proliferation-inhibitory factor(s)act on the early events of T lymphocyte acti-vation induced by Con A.(3)Protein kinase C(PKC)is a key link in the activa-tion of T and B lymphocyte proliferation by Con A and LPS respectively.So itwould be interesting to learn whether the inhibitory effect of the lymphocyte pro-liferation-inhibitory factior(s)is caused by the inhibition of PKC activity.It wasobserved that the serum indeed inhibits the lymphocyte proliferation induced byPMA(a PKC activator)or in combination with Ca^(2+)ionophore A23187.Theseresults suggest that the inhibitory effect of the lymphocyte proliferation-inhibitoryfactor(s)might be mediated by some direct inhibition of PKC activity or indi-rectly through PKG activated pathways.