摘要
本文利用免疫荧光技术,用角蛋白和波形蛋白抗体作为组化探针,对不同年龄大鼠的整体和离体培养的附睾上皮细胞进行鉴定,区分出结缔组织成分。并证明用0.05%胰蛋白酶和0.1%胶原酶相继消化附睾管可得到较丰富的上皮细胞,体外培养能维持10d 以上,在培养过程中显示其形态特征。因此本文所用的分离、培养方法所得到的附睾上皮细胞可作为深入研究其功能的一种模型。
The antibodies of keratin and vimentin were used as the histochemical probesdetermined by the immuno-fluorescent technique to recognize the rat epididymalepithelial cells in the different ages from the connective tissue both in intact epidi-dymides and in isolated cultured cells.It also showed that an enriched suspensionof epididymal epithelial cells could be obtained by sequential digestion with 0.05%trypsin and 0.1% collagenase.The morphological characteristics were appearedduring the cells in culture.Therefore the epididymal epithelial cells isolated andcultured by present methods could be used as a research model to study their fun-ctions.
出处
《生理学报》
CAS
CSCD
北大核心
1990年第6期578-581,共4页
Acta Physiologica Sinica
关键词
大鼠
附睾上皮细胞
epididymal epithelial cells
intermediate filaments
keratin
vimentin
rat
