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多重PCR法检测食品中大肠杆菌O157 被引量：1

A multiplex PCR system for the detection of Escherichia coli O157 in food
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摘要 以大肠杆菌O157的stx1、stx2、wzy基因为靶基因,建立了一种新的多重PCR检测方法。PCR扩增结果与各参考菌株基因型一致。PCR结果表明,wzy基因特异性强,可作为检测O157菌株的标志基因,且为开发大肠杆菌O157的分子检测技术提供新的研究思路。 A new multiplex PCR was developed to detect Escherichia coli O157 by the amplification of stx1,stx2 and wzy genes.It detected the presence of the three target genes in a manner consistent with the known genotype of each reference strain.The PCR amplification result demonstrated that wzy gene was O157 serotype specific,and could be used for the differentiation of O157 and non-O157 serotypes.This study offered a new method for the detection of Escherichia coli O157.
作者 李睿 戴诗皎 杨敬镜 瞿敏
机构地区 武汉工业学院生物与制药工程学院
出处 《武汉工业学院学报》 CAS 2010年第3期1-3,共3页 Journal of Wuhan Polytechnic University
关键词 大肠杆菌O157 多重PCR stx1 stx2 wzy Escherichia coli O157 multiplex PCR stx1 stx2 wzy
分类号 R155.5 [医药卫生—营养与食品卫生学]
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参考文献7

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二级参考文献38

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共引文献22

同被引文献7

  • 1中华人民共和国卫生部.全国肠出血性大肠杆菌0157:H7感染性腹泻应急处理预案(试行)[S].北京:中华人民共和国卫生部,2002.
  • 2Fukushima H, Kawase J, Etoh Y, etal. Simultaneous screening of 24 target genes of foodborne pathogens in 35 foodbome out- breaks using multiplex real-time SYBR green PCR analysis[J]. Int J Mierobiol, 2010 ,Pii: 864-817.
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  • 5宾羽琳,陈艳,邱灿林.胶体金免疫层析法应用于感染性肠道疾病病原的快速检测[J].中国卫生检验杂志,2008,18(5):854-856. 被引量：4
  • 6雷永良,叶碧峰,梅建华,柳付明,陈莎彬,兰进权,李永芬,陈秀英.实时荧光定量PCR技术在群体感染性腹泻病原体快速筛查中的应用[J].中国卫生检验杂志,2008,18(6):1000-1003. 被引量：8
  • 7钟珍,齐晖,李富荣,周汉新,蒋锦杏.胶体金免疫层析法检测大肠杆菌O157:H7的实验研究[J].实用医学杂志,2010,26(13):2280-2283. 被引量：8

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武汉工业学院学报
2010年 第3期

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