摘要
进行了用次氨酸钠与氯仿技体积比1:1的混合液从重组大肠杆菌中回收生物可降解塑料PHA的研究，发现次氯酸钠可快速将细胞破壁，同时PHA分子可及时溶入氯仿中以防止分子量的过度降低．实验结果表明，当混合液中次氯酸钠的体积浓度为20％，反应时间为15min时提取出的PHA纯度可达90％，当次氯酸钠的体积浓度为30％～40％时PHA提取率约为70％．
Using dispersion of sodium hypochlorite solution and chloroform (1:1) toextract PHA from microorganism was studied. The initial PHA content in the microorganismwas 60% (w). Experimental results show that 15 min are enough for PHA recovery fromdisrupted cells. Chloroform can protect PHA from further degradation by sodium hypochloritesolution. When the sodium hypochlorite volume concentration is 20% and reaction time is 15minutes, the purity of the recovered PHA reaches 90%. The recovery ratio is about 70% whenusing 30%～40% sodium hypochlorite.
出处
《化工冶金》
CSCD
北大核心
1999年第3期286-289,共4页
关键词
生物可降解塑料
PHA
提取
次氯酸钠
氯仿
Biodegradable plastic
Extraction
Cell disruption
Molecular weight
