2株枯草芽孢杆菌对梨轮纹病菌的室内抑制作用研究

Inhibition effect of 2 Bacillus subtilis on Botryosphaeria berengeriana f. sp. piricola in vitro

研究了2株枯草芽孢杆菌Sf-19和Sf-28对梨轮纹病菌的室内抑制作用,结果表明,枯草芽孢杆菌的不同浓度处理对梨轮纹病菌菌丝生长、孢子萌发及果实上梨轮纹病菌均有一定的抑制作用。枯草芽孢杆菌Sf-19培养原液对梨轮纹病菌的综合抑制效果最佳,对梨轮纹病菌菌丝生长的平板抑制率为87.33%,孢子萌发的抑制率为97%,果实上梨轮纹病菌的抑制效果研究分为保护(Ⅰ)和治疗(Ⅱ)两部分试验进行,Sf-19培养原液保护试验(Ⅰ)中对梨轮纹病菌的抑制率为71.97%,治疗试验(Ⅱ)为64.92%;生防菌Sf-28培养原液对梨轮纹病菌的综合抑制效果次之,对梨轮纹病菌菌丝生长的平板抑制率为87%,孢子萌发的抑制率为80%,Sf-28培养原液对果实上梨轮纹病菌的抑制率保护试验(Ⅰ)为64.54%,治疗试验(Ⅱ)为59.21%。2株枯草芽孢杆菌均随着稀释倍数的增大防治效果降低,综合比较枯草芽孢杆菌Sf-19、Sf-28不同浓度处理的效果好于对照生防菌株Bs-916。

The inhibition effect of 2 Bacillus subtilis Sf-19 and Sf-28 on Botryosphaeria berengeriana f.sp. piricola by was studied in vitro. The results showed that： 2 Bacillus subtilis Sf-19 and Sf-28 at different concentrations had certain extent inhibition effects on mycelium growth, spore germination and pathogen inoculated on pear fruits ofBotryosphaeria berengeriana f. sp. piricola. Stock solution of Sf-19 had the best comprehensive inhibition effect , and the inhibition rate on mycelium growth and on spore germination was 87.33%, and 97%, respectively. The study of the inhibition rate on Botryosphaeria berengeriana f.sp. piricola inoculated on pear fruits was divided into two parts： protection test and therapeutic experiment. In protection test（ Ⅰ ）, the inhibition rate of stock solution of Sf-19 was 71.97%, and in therapeutic experiment （Ⅱ）was 64.92%; stock solution of Sf-28 had the better comprehensive inhibition effect on Botryosphaeria berengeriana f.sp. piricola, the inhi- bition rate on mycelium growth and spore germination on PSA plates was 87% ,and 80%, respectively. In protection test （ Ⅰ）, the inhibition rate of stock solution of Sf-28 was 64.54%, and in therapeutic experiment （Ⅱ）was 59.21%. With the increase of dilution times of Bacillus subtilis, the inhibition effect decreased. Comprehensive comparison among 3 Bacillus subtilis, the inhibition effect of Sf-19 and Sf-28 was better than that of CK Bs-916.