IL-17A基因-121G/A多态性与中国北方汉族人群早发冠心病的关系

Association of IL-17A-121G/A polymorphism and premature coronary artery disease in Han population of North China

目的探讨IL-17A基因-121G/A（IL-17A）单核苷酸多态性与中国北方汉族人群早发冠心病（CAD）发病的相关关系。方法采用聚合酶链反应—重测序法检测经冠状动脉造影证实的520例早发CAD患者和480例非CAD对照者IL-17A基因-121G/A单核苷酸多态位点的基因型和等位基因分布情况。结果 IL-17A基因-121G/A单核苷酸多态三种基因型（GG型、GA型和AA型）在早发CAD患者中的分布频率分别为86.9%、12.2%和0.9%,在对照者分别为81.2%、17.8%和1.0%,两组间的基因型分布皆符合Hardy-W e inberg平衡定律,IL-17A基因-121G/A单核苷酸多态的GG基因型和G等位基因分布频率在两者间存在统计学差异（OR=0.68;95%可信区间为0.49-0.94;P=0.02）。按性别进行亚组分析,IL-17A基因-121G/A单核苷酸多态的GG基因型和G等位基因频率在男性CAD和对照者之间存在统计学差异（OR=1.56;95%可信区间为1.04-2.28;P=0.02）。Logistic回归分析显示,IL-17A基因-121G/A多态性是CAD发病的独立危险因素（P〈0.05）。结论中国北方汉族人群中存在IL-17A基因-121G/A单核苷酸多态性。IL-17A基因-121G/A单核苷酸多态性与早发CAD的发病存在相关关系,G等位基因携带者发生CAD的危险性增加。

Objective To assess the possible association between interleukin17A（IL-17A） and premature coronary artery disease（CAD）.Methods A total of 520 patients with CAD and 480 healthy adults were genotyped by polymerase chain reaction and DNA sequence technology for the-121A/G polymorphism of IL-17A gene.Results There was IL-17A gene-121A/G polymorphism in Northern Chinese Han population.The genotype frequencies of GG,AG and AA in the IL-17A-121G/A polymorphism were 86.9%,12.2% and 0.9% in CAD,81.2%,17.8% and 1.0% in the controls respectively.The frequencies of GG genotype and G allele were significantly higher in patients with CAD than those of controls（OR=0.68,95% CI=0.49~0.94,P=0.02）.Subsequent stratified analysis by gender also showed there was a statistical significance in the genotype and allele frequencies between the male patients and control group（OR=1.56,95% CI=1.04~2.28,P=0.02）.A binary logistical regression analysis suggested the IL-17A-121G/A polymorphism correlated with CAD（P〈0.05）.Conclusions IL-17A gene-121G/A polymorphism associates with the risk of CAD,and G allele carriers may be a possible genetic susceptibility factor for patients with CAD in Northern Chinese Han population.