白细胞介素1αC(-889)T单核苷酸多态性与冠心病的相关性

A single nucleotide polymorphism of C(-889)T in interleukin-1α associated with coronary artery disease

目的研究白细胞介素1α(IL-1α)基因启动区上游C(-889)T单核苷酸多态位点与中国人群冠心病的相关性。方法采用聚合酶链反应—限制性片段长度多态性方法检测186例冠心病患者及170例健康对照者的IL-1αC(-889)T基因型,比较两者基因型及等位基因频率分布,以及不同基因型对血脂参数、高敏C反应蛋白(hs-CRP)及冠脉受累支数的影响。结果与健康对照比较,冠心病患者IL-1αC(-889)T的CC基因型频率降低,CT基因型频率及T等位基因频率明显升高(P均<0.05)。冠脉受累支数与基因型无关。未发现基因型与血脂参数相关,但CT+TT基因型hs-CRP水平升高(P<0.05)。结论 IL-1αC(-889)T单核苷酸多态性与中国人群冠心病相关,T等位基因是冠心病的遗传易患因子。

Objective To detect the association of single nuclear polymorphism（SNP） of C（-889）T in the interleukin-1α（IL-1α） gene with coronary artery disease（CAD） susceptibility in Chinese.Methods IL-1α genotypes of 186 cases of CAD and 170 cases of healthy control were detected by PCR-RFLP.Differences in frequencies of IL-1α genotypes and alleles and their associations with clinical features were assessed.Results The frequencies of CC genotype in CAD group were lower than those in control subjects,while CT genotype and T allle in CAD group were higher than those in control subjects（all P〈0.05）.No statistical difference was observed between IL-1αC（-889）T polymorphism and severity of the coronary lesions.There were no significant differences among genotypes on serum levels of lipids in either CAD group or control group.However,CT＋TT genotypes had higher serum levels of hs-CRP in both CAD group and healthy control.Conclusions Polymorphism of C（889）T in IL-1α gene associates with CAD,T allele is probably a genetic marker of CAD in Chinese.