PG490在EAE中的靶向作用机理研究

THE TARGET FUNCTION OF PG490 IN EAE AND RESEARCH FOR ITS MECHANISM

目的探讨PG490在EAE中的干预作用及作用途径。方法把45只SD大鼠随机分为三组即正常对照组、模型组及PG490干预组。分别采用免疫组化及ELISA的方法观察NF-κBp65及COX-2的含量变化,同时观察干预前后临床学评分及病理变化。结果 PG490干预后模型鼠临床评分及病理学的变化均较干预前有所改善;干预前后NF-κBp65及COX-2的含量变化相比较有显著性差异(p<0.05)。结论 PG490能够减轻EAE模型的临床症状,其作用机理可能为通过产生抑制性细胞因子,进而影响NF-κBp65及COX-2等炎症途径效应。

Objective To investigate the target function of PG490 and its mechanism in EAE.Methods Forty-five SD rats were classified by control,EAE and PG490 groups randomly.The clinical scores,pathological changes and expression and content of NF-κBp65 and COX-2 were tested by immuno-histochemistry and ELISA before and after treatment.Results There are significant improvement about clinical scores and pathological changes after intervention; There is significant difference about activity of NF-κBp65 and the content of COX-2 between EAE group and PG490 group.Conclusion The role of PG490 is obvious.PG490 can alleviate clinical symptoms of EAE by suppressing inflammatory and immunological reaction and interfering the pathway for NF-κBp65 activation in EAE.