过敏性哮喘患者外周血中IL-17和IL-23表达水平的变化及其临床意义

Expression Levels and Clinical Significance of IL-17 and IL-23 in Peripheral Blood of Patients with Allergic Asthma

目的探讨过敏性哮喘患者外周血中白细胞介素(interleukin,IL)-17和IL-23表达水平的变化及其与疾病严重程度的相关性。方法采用ELISA方法检测过敏性哮喘患者血浆中以及外周血单个核细胞(peripheral blood mono-nuclear cells,PBMCs)培养液上清中IL-17和IL-23的水平,并分析这两个炎症因子与疾病严重程度的相关性。结果过敏性哮喘患者血浆中以及PBMCs培养液上清中IL-17、IL-23的水平均较健康对照组升高(P<0.05),且血浆中IL-17的水平随哮喘的严重程度增加而升高。血浆中与PBMCs培养液上清中IL-17与IL-23水平均呈正相关(r=0.526,P=0.003;r=0.428,P=0.021)。结论 IL-17和IL-23参与了哮喘的发生发展,血浆中的IL-17水平也许可以作为判断哮喘严重程度的一个参考指标。

Objective To investigate the expression levels of IL-17 and IL-23 in patients with asthma and analyze their correlation with the severity of asthma. Methods The levels of IL-17 and IL-23 in plasma and those in culture supernatants of peripheral blood mononuclear cells（PBMCs）were measured by enzyme linked immunosorbent assay（ELISA）and their correlation with the severity of disease was analyzed. Results The levels of IL-17 and IL-23 in plasma and culture supernatants of PBMCs were significantly elevated in patients with asthma as compared with healthy controls（P〈0.05）and IL-17 levels in plasma were increased with disease severity. The levels of IL-17 were correlated with those of IL-23 in both plasma and culture supernatants of PBMCs（r= 0. 526, P = 0. 003 and r=0. 428, P = 0.021, respectively）. Conclusion IL-17 and IL-23 play a role in the pathogenesis of allergic asthma. The plasma level of IL-17 may be a marker for the severity of asthma.