海狗油脂肪乳对大鼠围产期毒性试验的研究

The Toxic Effects of Seal Oil Emulsion on Rats during Perinatal Period

观察了海狗油脂肪乳对大鼠妊娠晚期、分娩期、哺乳期及胚胎和胎仔出生后生长发育、学习能力以及生殖能力的影响.于大鼠妊娠D15至哺乳D21,连续皮下注射给予250、500和1000mg·kg-1剂量的海狗油脂肪乳,同时设0.9%氯化钠注射液为对照组,观察各组大鼠和胎仔的生长、发育、生殖能力等项指标.结果表明:给药后F0代母鼠体重增长明显减慢,摄食量显著减少,未见生殖、胚胎毒性反应;F1代仔鼠的出生体重和哺乳期体重增长减慢,生理达标、新生反射等发育迟缓;停药后F1代大鼠的体重增长、摄食量、脏器系数、同笼交配率、妊娠率及胚胎发育全过程未见明显生殖、发育等毒性变化.由此结论,海狗油脂肪乳可使母鼠和F1代仔鼠体重增长缓慢,生理发育迟缓,但不影响F1代仔鼠器官发育、生殖能力以及停药后体重增长和摄食量.

In order to observe the toxic effects of Seal Oil Emulsion on the maternal rat in late trimester of pregnancy,the growth and development,and the abilities of learning and procreation of the offsprings,SD pregnant rats were given Seal Oil Emulsion orally from gestational day 15 to weaning day 21.The experimental groups received 250、500 and 1000mg·kg-1 of Seal Oil Emulsion.Results showed that the body weight accretion and appetite of maternal rat（F0）in experimental groups was significantly lower than that in negative control group（p 0.05）.The body-weight of the offsprings（F1）at birth and accretion during lactation were significantly decreased（p0.05）.The physiological indexes and neonatal reflexes were delayed significantly（p0.05）.But there were no toxic effects on the body weight gain,appetite,visceral measurements,mating ratio,pregnancy ratio and fetal development on stopping drug administration.It was indicated that among the range used,Seal Oil Emulsion caused obvious body weight accretion in F0 and F1,and developmental delay.But there were no the toxic effects on the visceral development,reproductive ability,body weight increase,and appetite on stopping the drug.