天竺桂叶精油成分分析及其对蚊虫的毒杀作用

Component analysis of essential oil from fresh leaves of Cinnamomum japonicum and oil's activity to mosquitoes

用水蒸汽蒸馏法从天竺桂鲜叶中提取精油,用GC-MS(Gas Chromatography-Mass Spectrometry)检测其化学成分,采用浸液法研究其对白纹伊蚊和致倦库蚊的毒杀活性。结果表明:天竺桂叶精油中,化合物相对含量最高的成分为2—莰醇(28.30%),其它依次为桉叶油醇(20.98%)、乙酸龙脑酯(6.65%)、芳樟醇(5.94%)、α—松油醇(5.87%)、(-)-环氧石竹烯(5.11%)等;天竺桂精油对白纹伊蚊Ⅳ龄期幼虫和蛹的LC50值分别为75.466、87.508μg/mL,而对致倦库蚊则分别是61.804、77.654μg/mL。结果表明天竺桂叶精油对这2种蚊虫的幼虫和蛹均有显著的毒杀效果。

The essential oil was extracted from Cinnamomum japonicum fresh leaves by steam distillation,the compositions of this oil was analyzed by gas chromatography-mass spectrometry,and the toxic activity of this oil against 4th larvae and pupae of Aedes albopictus and Culex pipiens quinquefasciatus were determined by immersion method.The main components of the essential oil were Borneoll（28.30%）,1,8-Cineole（20.98%）,（-）-Bornyl Acetate（6.65%）,Linalool（5.94%）,（-）-Alpha.-Terpinol（5.87%）,Caryophyllene Oxlde（5.11%）.The results show that the toxicity of LC95 against 4th instar larvae and pupae of Ae.albopictus were 75.466 μg/mL,87.508 g/mL,respectively,and the toxicity of LC95 to 4th instar larvae and pupae of Cx.quinquefasciatus were 61.804 μg/mL and 77.654 μg/mL.So,a conclusion is drawn that the essential oil has obvious toxic effect to both larvae and pupae of the two mosquito species.