体外筛选具有抗柯萨奇病毒作用的中药提取物

Screening of traditional Chinese herbal extracts against Coxsackie virus in vitro

目的通过建立体外抗病毒药物筛选平台,寻找具有抗柯萨奇病毒的中药。方法根据文献报道,候选39种可能具有抗病毒作用的中药,对其有效部位进行水体物和醇提物,共获得160种中药有效部位。培养Hela细胞,接种病毒和药物,通过以噻唑蓝(MTT)染色方法为主的细胞病变抑制实验和病毒繁殖抑制实验对上述药物进行筛选,之后通过公式计算有明确抗病毒作用药物的半数有效剂量(IC50)和半数致死量(CC50)。结果在候选药物的有效部位中,黄芪甲苷和红景天苷具有显著抗病毒作用,细胞病变(CPE)抑制实验结果显示黄芪甲苷的半数有效量(IC50)为(6.4±0.5)μg/ml、红景天苷IC50值为:(9.6±1.4)μg/ml;黄芪甲苷半数毒性剂量(CC50)为(1287±182)μg/ml、红景天苷CC50值为:(952±83)μg/ml。2种提取物的的治疗指数(SI)分别为:200和99。结论黄芪水体物和醇提物、红景天醇提物具有抗病毒作用,其细胞毒性均低于对照药利巴韦林,其中黄芪醇提物的体外抗病毒效果与利巴韦林接近。

Objective To screen for new antiviral agents from traditional Chinese medicine specific against Coxsackie virus. Methods According to literatures, 39 types of herbs considered to be potentially antiviral agents were subjected to water or alcohol extraction of their active parts, thereby producing 160 extracts. Hela cell culture was used to inoculate Coxackie virus and then added with study extracts. Inhibition of cytopathic effect （CPE） and virus proliferation were measured with a MTT-based eolofimetfic assay. The IC50 and CC50 of promising herbs were calculated by the results of MTT assay. Results Among the 160 extracts, Astragalus and Salidroside exhibited significant antiviral activity. Inhibition test of CPE showed, the IC50 and CC50 was （6.4±0.5） μg/ml and （1287±182）μg/ml for Astra- galus, and was （9.6±1.4） μg/nd and （952±83） μg/ml for Salidroside. Based on the formula of CC50/IC50, selectivity index of Astragalus and Salidroside was 200 and 99, respectively. Conclusion Astragalus and Salidmside showed antiviral effect against Coxsackie virus with less cytotoxity as compared with Ribavirin. The in-vitro antiviral effects of water extract of Astragalus was comparable to Ribavirin.