缺血前运动训练对小鼠脑局灶缺血后功能恢复的影响

Effects of preoperative wheel running train on the functional outcome after focal brain infarction in mice

目的：探讨在大脑中动脉闭塞前进行运动训练，是否有利于小鼠脑局灶缺血后的功能恢复。方法：选雄性Ｃ５７ＢＬ／６Ｊ小鼠２３只，随机分两组；缺血前运动组（Ａ，ｎ＝１１）和不运动对照组（Ｂ，ｎ＝１２），运动组每天跑笼训练１小时，连续１４天后两组均电凝右侧大脑中动脉，制成脑梗塞模型，在其后３、７、１４天观察动物的行为变化，并在术后１４天，取脑，进行尼氏染色，观察梗塞体积。结果：缺血前运动组较对照组功能恢复快（Ｐ＜０．０１）；两组梗塞体积，运动组小于对照组（Ｐ＜０．０５）；运动组行为变化与梗塞体积成负相关（Ｐ＜０．０５）。结论：缺血前运动训练有助于小鼠脑局灶缺血后的功能恢复，并能减少梗塞灶。

Objective:To determine whether preoperative wheel runninng training can enhance the functional outcome after cerebral infarction in mice.Method:Male C57BL/6J mice were trained in running wheel for 14 days as physical activity group (A,n=11), control group mice (B,n=12) stayed in the normal cages. All the mice were underwent the right middle cerebral artery occlusion (MCAO) with electric coagulation.A battery of three tests that assessed muscle strengh,posture reflex and balance were performed at day 3,7,14, and infarct volume was calculated at day 14 after MCAO. Result:Group A performed significantly better than group B in motor behavior( P <0.01), after MCAO at day 3,7. Infarct volume in group A was smaller than that in group B ( P <0.05). The relationship between infarct volume and behavior were negatively related in group A (P <0.05) but not in Group B ( P >0.05) at day 14 after MCAO. Conclusion:The preoperative wheel running train can enhance the functional outcome and reduce infarct volume.