漂洗对脂肪活性的影响

Effect of Rising on Fat Viability

目的比较不同漂洗液和不同漂洗次数对吸脂术所获得的脂肪组织活性的影响。方法通过肿胀吸脂术获得脂肪组织后,分别用生理盐水、平衡液各漂洗0、1、3、5、10次后,用葡萄糖转移实验检测其活性。结果生理盐水组脂肪组织的葡萄糖转移量大于平衡液组,差异具有统计学意义(P<0.05);两组中,漂洗0和1次时的葡萄糖转移量明显低于漂洗3次(P<0.01);生理盐水组中,漂洗3次和5次时的葡萄糖转移量无明显差异(P>0.05),但漂洗10次时的葡萄糖转移量明显低于漂洗3次和5次(P<0.05);平衡液组中,漂洗3次、5次和10次时的葡萄糖转移量无明显差异(P>0.05)。结论通过漂洗可以提高吸脂术所获得的脂肪组织的活性。本实验中,生理盐水的漂洗效果优于平衡液,以漂洗3次为佳。

Objective To investigate the effect of rinsing with different fluids and times on fat viability in autologous fat transfer.Methods Fat was obtained by tumescent liposuction and then rinsed by normal saline or balanced fluid for 0,1,3,5,and 10 times.Fat viability was determined by glucose transportation test.Results The number of glucose transportation was higher in normal saline group,as compared with that in balanced fluid group（P〈0.05）.In both the groups,the number of glucose transportation in 3-time rinsing subgroup was lower than 0-and 1-time rinsing subgroups（P〈0.01）.In normal saline group,the number of glucose transportation showed no statistical difference between 3-time and 5-time rinsing subgroups（P〉0.05）,and was lower in 10-time rinsing subgroup as compared with 3-time and 5-time rinsing subgroups（P 〉0.05）.In balanced fluid group,there were no significant differences in the number of glucose transportation between 3-,5and 10-time rinsing subgroups（P 0.05）.Conclusion Rinsing can improve the viability of fat following tumescent anesthesia.The increase in fat viability will be higher with normal saline rinsing,as compared with balanced fluid rinsing.Fat should be fully rinsed for 3 times with normal saline.